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简单实验流程，节约时间和成本

1小时3步组装具有编辑功能的Alt-R® RNP

多种方式高效导入Alt-R® RNP

提供针对不同模式生物的实验方法

Alt-R® RNP可在体外对DNA进行编辑


优化的gRNA —Alt-R® crRNA : tracrRNA复合体

crRNA: tracrRNA 优化长度显著改善编辑效率

特殊修饰crRNA:tracrRNA 增加核酸酶抗性降低免疫反应

多物种在线crRNA预设计数据库及检查器


“千挑万选”高保真Cas9酶 —Alt-R® S.P. HIFI Cas9

对目标位点较高的编辑效率

基因编辑脱靶效应显著降低

与gRNA复合体形成RNP，细胞毒性低，不易引起免疫反应

怎样的神操作 60分钟进行CRISPR 基因编辑>>

 

人类干细胞上的测试结果表明，与其他高保真Cas9酶比， IDT新高保真Cas9酶呈现出稳定的中靶编辑活性，脱靶率又极低。我们被IDT新高保真Cas9酶的表现所折服，已在使用它来开发基于基因编辑的疾病治疗方案。
--马修·波特斯（Matthew Porteus） 博士，干细胞移植专家，美国斯坦福大学

文献列表

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  • di Pietro F, Valon L, et al..?(2017)?An RNAi screen in a novel model of oriented divisions identifies the actin-capping protein Z β as an essential regulator of spindle orientation.?Curr Biol,?27?:?2452–2464.
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  • Quadros RM, Miura H, et al.?(2017)?Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins?.?Genome Biology,?18?:?92.
  • Wefers B, Bashir S, et al.?(2017)?Gene editing in mouse zygotes using the CRISPR/Cas9 system.?Methods,?121–122?:?55–67.
  • Rivera-Torres N, Banas K, et al..?(2017)?Insertional mutagenesis by CRISPR/Cas9 ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides?.?PLoS One,?12?:?e0169350.
  • Agudelo D, Duringer A, et al..?(2017)?Marker-free coselection for CRISPR-driven genome editing in human cells.?Nature Methods,?14?:615–620.
  • Luo L, Bokil N, et al..?(2017)?SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages?.?Nat Commun,?8?:?14133.
  • Cuellar TL, Herzner AM, et al.?(2017)?Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.?J Cell Biol,?216?:?3535–3549.
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